Pituitary tumor transforming gene 1(PTTG1)가 구강 편평상피세포암 이동에 미치는 영향
Effect of Pituitary Tumor Transforming Gene 1 in Oral Squamous Cell Carcinoma for Migration
이상신, 김진, 이석근,
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이상신 ( Lee Sang-Shin ) - 강릉원주대학교 치과대학 구강병리학교실
김진 ( Kim Jin ) - 연세대학교 치과대학 구강병리학교실
이석근 ( Lee Suk-Keun ) - 강릉원주대학교 치과대학 구강병리학교실
KMID : 0829220110350030187
Abstract
Human pituitary tumor transforming gene 1 (PTTG1), which is a newly identified proto-oncogene, is highly expressed in normal pituitary tissues containing proliferating cells and in several cancers. Also, PTTG1 has been known as a securin to involve in the regulation of cell-cycle and in the progression of tumor. But the effect of PTTG1 in oral squamous cell carcinoma (oral SCC) has not been studied yet. The objective of this study is to analyze the expression of PTTG1 in oral SCC cell lines (YD-10B and YD-15) and to evaluate the effect of PTTG1 on oral SCC cell lines for the migration effect by PTTG1 siRNA treatment. Western blot, migration assay, and zymography were performed to evaluate the effects of PTTG1 on the expression of MMP-2/-9 and migration activity after PTTG1 siRNA treatment. PTTG1 was expressed in oral SCC cells lines, otherwise, significantly decreased after PTTG1 siRNA treatment. There is no difference in expression of MMP-2 regardless of PTTG1 siRNA treatment. However, the enzyme activity of MMP-9 was significantly decreased. In addition, the migration activities of oral SCC cells were significantly decreased after PTTG1 siRNA treatment (p＜0.001). These results suggested that the down-regulated PTTG1 could inhibit the migration of human oral SCC cells through the low MMP-9 expressions. Therefore, these findings provide a useful guideline for the migration mechanism of oral SCC depend on PTTG1 expression.
키워드
Integrin; Invasion; Oral squamous carcinoma cell; Pituitary tumor transforming gene 1; siRNA
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